Technology in medicine has taken a new dimension. With regards to testing for presence of diseases and other anomalies, Elisa technology has taken over. The cardiac Elisa kits have been particularly so good. They are devices capable of working with the hearts of almost all animals in the world to establish any defects on it.
The process of diagnosing illnesses depends on an enzyme reaction that gives its results through exhibiting certain color changes. The enzyme and some antibodies combine with antigens, and subsequently change their color to indicate occurrence of a reaction. With this test, researchers are able to detect both antigens and antibodies.
This process can be used in establishing the presence of foreign bodies in human beings. It is very important since it helps in detecting and treating heart problems before they develop into serious problems. This helps in cutting down the cost involved in diagnosing and treating heart defects. This is because the defects are discovered in their early stages before they become serious problems.
The best of these tools is one that is accurate, sensitive and capable of working in a wide range of samples. Sensitivity means being able to detect and show slight changes in the reagents. Accuracy, on the other hand, is being free of major errors in making measurements. It is also very important for the instrument to be made in a way such that, each heart defect has its own diagnostic equipment.
The device should also be stable. This is achieved through reducing the loss rate as much as may be possible. The tools should be stored in good conditions to ensure that they remain stable. Other environmental influences should be completely avoided. Appropriate environmental conditions should, therefore, be provided. These include; appropriate temperature, pressure and humidity. Somebody should be given the responsibility of controlling the temperature in the incubator at all times. Assigning one person to work on the experiment is also crucial in ensuring stability.
For proper working of this activity, the researcher must ensure that all standards, reagents and samples are prepared in advance. The next thing is addition of some samples to all the wells and incubating them for close to 2 hours. He should then aspire and add some reagents. Next, he should put it back in the incubator for an hour, and later aspire and wash the mixture three times. The substrate solution should then be added, and the mixture incubated for a period of between 20 and 25 minutes. After, this step, a stopping agent must be added to stop the reaction for the researcher to make readings.
The main principle applied here is enzyme sandwich. The plates inside the testing equipment are always coated with antibodies for specific heart defects. Samples are put into the plates in an appropriate manner. The main contents of the samples are specific biotin-conjugate antibodies. Before incubating, a conjugate of Avidin is added to all plates.
After putting substrate solutions together with other reagents, only the micro-wells will have Tropin I type three. A color change will then be exhibited, and a stopper solution is added. The change in color is then measured using wavelengths.
The process of diagnosing illnesses depends on an enzyme reaction that gives its results through exhibiting certain color changes. The enzyme and some antibodies combine with antigens, and subsequently change their color to indicate occurrence of a reaction. With this test, researchers are able to detect both antigens and antibodies.
This process can be used in establishing the presence of foreign bodies in human beings. It is very important since it helps in detecting and treating heart problems before they develop into serious problems. This helps in cutting down the cost involved in diagnosing and treating heart defects. This is because the defects are discovered in their early stages before they become serious problems.
The best of these tools is one that is accurate, sensitive and capable of working in a wide range of samples. Sensitivity means being able to detect and show slight changes in the reagents. Accuracy, on the other hand, is being free of major errors in making measurements. It is also very important for the instrument to be made in a way such that, each heart defect has its own diagnostic equipment.
The device should also be stable. This is achieved through reducing the loss rate as much as may be possible. The tools should be stored in good conditions to ensure that they remain stable. Other environmental influences should be completely avoided. Appropriate environmental conditions should, therefore, be provided. These include; appropriate temperature, pressure and humidity. Somebody should be given the responsibility of controlling the temperature in the incubator at all times. Assigning one person to work on the experiment is also crucial in ensuring stability.
For proper working of this activity, the researcher must ensure that all standards, reagents and samples are prepared in advance. The next thing is addition of some samples to all the wells and incubating them for close to 2 hours. He should then aspire and add some reagents. Next, he should put it back in the incubator for an hour, and later aspire and wash the mixture three times. The substrate solution should then be added, and the mixture incubated for a period of between 20 and 25 minutes. After, this step, a stopping agent must be added to stop the reaction for the researcher to make readings.
The main principle applied here is enzyme sandwich. The plates inside the testing equipment are always coated with antibodies for specific heart defects. Samples are put into the plates in an appropriate manner. The main contents of the samples are specific biotin-conjugate antibodies. Before incubating, a conjugate of Avidin is added to all plates.
After putting substrate solutions together with other reagents, only the micro-wells will have Tropin I type three. A color change will then be exhibited, and a stopper solution is added. The change in color is then measured using wavelengths.
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