ELISA is short for enzyme linked immunosorbent assay. This is a diagnostic medical test to see if there is any present antibody or antigen in the body of a person. Its purpose is for calibrating the immunity of a person in terms of diseases and viruses. It identifies the protein concentration in the bodily fluids of a person.
An example for this would be for HIV testing. It looks for the certain antibodies which is related to the HIV virus within the blood. In regards to the thyroid glands, what is used are Thyroid ELISA kits, which are very common. Other testing kits are available as well for other parts of the body.
Finding out if you have any specific food allergy is also done using this test. It may be used for testing if a patient is using illegal drugs. Generally, it is a tool for plant pathology and quality control check for certain industries. The calibration or reading is through how vibrant the color is and its changes after each sample is tested.
There are a few other ELISA tests that are available and existent, but the two common types are the indirect and capture. The indirect detects a certain protein or antibody, and an example for this is the HIV testing. The capture, also known as the sandwich, detects another protein which is the antigen, then captures it between two antibodies. An example is pregnancy tests where the detection of hCG or human chorionic gonadotropin is seen.
There are many ways to collect samples from the patient or donor. Two common ways are from blood or urine, which by now, you have already guessed, is taken during tests in clinics and hospitals. For blood samples, they are placed inside a test tube and sent to the laboratories for further analysis. Inside the laboratory, the actual testing would begin.
A centrifuge is used to make a blood serum which is used during the tests. The centrifuge would separate the compositions of the blood which are the cells and the plasma. A blood serum is the actual sample used in tests, and the clotting feature is actually taken out already.
Horseradish Peroxidase is an enzyme mostly used for detection where it separates Ortho Phenylenediamine Dihydrochloride and Tetramethylbenzidine. It results into a yellow color and for measuring the optical density, a plate reader would then be used. Light absorbance from OPD is 490 nanometers in wavelength, while TMB is at 450 nanometers.
If the patient has already been determined to have a disease or condition, the samples of their blood or urine will have certain antibodies which will be the ones to react to the test. These antibodies will stick to the antigens, which these antigens are bonding agents for the entire test. A solution is used to wash away everything in it except for the antigens, or also the antibodies that are sticking to the antigens.
To get results through color changes, enzyme solutions would be added to the samples to get either a positive or a negative result. But there is a certain possibility for the test results to give a false positive. A false positive is when a sample has no infection or whatever but still gives a positive result. Even so, ELISA tests are reliable and considered to be a standard in the immunology community.
An example for this would be for HIV testing. It looks for the certain antibodies which is related to the HIV virus within the blood. In regards to the thyroid glands, what is used are Thyroid ELISA kits, which are very common. Other testing kits are available as well for other parts of the body.
Finding out if you have any specific food allergy is also done using this test. It may be used for testing if a patient is using illegal drugs. Generally, it is a tool for plant pathology and quality control check for certain industries. The calibration or reading is through how vibrant the color is and its changes after each sample is tested.
There are a few other ELISA tests that are available and existent, but the two common types are the indirect and capture. The indirect detects a certain protein or antibody, and an example for this is the HIV testing. The capture, also known as the sandwich, detects another protein which is the antigen, then captures it between two antibodies. An example is pregnancy tests where the detection of hCG or human chorionic gonadotropin is seen.
There are many ways to collect samples from the patient or donor. Two common ways are from blood or urine, which by now, you have already guessed, is taken during tests in clinics and hospitals. For blood samples, they are placed inside a test tube and sent to the laboratories for further analysis. Inside the laboratory, the actual testing would begin.
A centrifuge is used to make a blood serum which is used during the tests. The centrifuge would separate the compositions of the blood which are the cells and the plasma. A blood serum is the actual sample used in tests, and the clotting feature is actually taken out already.
Horseradish Peroxidase is an enzyme mostly used for detection where it separates Ortho Phenylenediamine Dihydrochloride and Tetramethylbenzidine. It results into a yellow color and for measuring the optical density, a plate reader would then be used. Light absorbance from OPD is 490 nanometers in wavelength, while TMB is at 450 nanometers.
If the patient has already been determined to have a disease or condition, the samples of their blood or urine will have certain antibodies which will be the ones to react to the test. These antibodies will stick to the antigens, which these antigens are bonding agents for the entire test. A solution is used to wash away everything in it except for the antigens, or also the antibodies that are sticking to the antigens.
To get results through color changes, enzyme solutions would be added to the samples to get either a positive or a negative result. But there is a certain possibility for the test results to give a false positive. A false positive is when a sample has no infection or whatever but still gives a positive result. Even so, ELISA tests are reliable and considered to be a standard in the immunology community.
About the Author:
Thyroid ELISA kits are now available to order online. We have all types of health test kits right here at http://www.elisatestkits.com.
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